Equipment

High Throughput automated planar patch clamp ideal for screening of modulators of ion channels and electrogenic transporters. Also useful for the functional characterization of complex cell populations. Cells must be in suspension to be used with this technique

MaxOne is a CMOS-based high density microelectrode array (HD-MEA), an electrical imaging system for neuroscience, drug discovery, and cell assessment applications. MaxOne captures high-quality signals at sub cellular resolution from acute tissue experiments and long-term culture experiments.

Key features

• 26,400 electrodes (9.3×5.45 sq-μm, 17.5 μm pitch)
• 1020 low-noise readout channels
• 32 stimulation channels
• Large sensor area (3.85×2.10 sq-mm)

Further details can be found here.

HEKA EPC10-USB amplifier

SliceScope Pro 1000 (Scientifica UK) with motorized XY stage

Scientifica SciCam Pro camera

GFP and mCherry / Texas Red Filter Sets

DIC condenser and IR ilumination

Suitable for:

Whole cell and single channel patch clamp

Patch clamp in tissue slices

GFP and mCherry -based imaging and cell identification

Axon CNS Multiclamp 700B

Axon CNS Digidata 1440A

Upright Olympus microscope BX51WI with FITC / EGFP filter

Leica DFC 350FX camera

Suitable for:

Whole cell patch clamp

Fluo-4 / EGFP-based imaging and cell identification

Suitable for measurement of epithelial membrane properties in tissue samples or cell monolayer grown in permeable support