Equipment

High Throughput automated planar patch clamp ideal for screening of modulators of ion channels and electrogenic transporters. Also useful for the functional characterization of complex cell populations. Cells must be in suspension to be used with this technique

The MaxOne and MaxTwo are single- and multi-well CMOS-based high density microelectrode array systems (HD-MEA). They are used for electrical imaging of excitable cells, drug discovery, and cell assessment applications. HD-MEAs capture high-quality signals at sub cellular resolution from long-term culture experiments (iPSC-neurons, primary neuronal cultures and cardiomyocytes) as well as from acute tissue experiments.
While the MaxOne system is more suited for acute tissue and organoid experiments (see mobile station atFees), the MaxTwo excells in cell culture experiments, where multple conditions can be cultured side-by-side in 6- or 24-well plates.

Key features

• 26,400 electrodes (9.3×5.45 sq-μm, 17.5 μm pitch)
• 1020 low-noise readout channels
• 32 stimulation channels
• Large sensor area (3.85×2.10 sq-mm)

Further details can be found here.

HEKA EPC10-USB amplifier

SliceScope Pro 1000 (Scientifica UK) with motorized XY stage

Scientifica SciCam Pro camera

GFP and mCherry / Texas Red Filter Sets

DIC condenser and IR ilumination

Suitable for:

Whole cell and single channel patch clamp

Patch clamp in tissue slices

GFP and mCherry -based imaging and cell identification

Dual headstage to measure connected cells in parallel
Dynamic clamp 
Integrated IgorPro software
Ratiometric live-imaging capabilities to study quantitative Calcium and Magnesium dynamics using pE-800-fura CoolLED 

Axon CNS Multiclamp 700B

Axon CNS Digidata 1440A

Upright Olympus microscope BX51WI with FITC / EGFP filter

Leica DFC 350FX camera

Suitable for:

Whole cell patch clamp

Fluo-4 / EGFP-based imaging and cell identification

Suitable for measurement of epithelial membrane properties in tissue samples or cell monolayer grown in permeable support